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egm 2 medium  (PromoCell)
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Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and <t>EGM-2.</t>
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Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and <t>EGM-2.</t>
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Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and <t>EGM-2.</t>
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Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and <t>EGM-2.</t>
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c 12203 egm  (PromoCell)
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Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and <t>EGM-2.</t>
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Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and <t>EGM-2.</t>
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Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and EGM-2.

Journal: Frontiers in Toxicology

Article Title: Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics

doi: 10.3389/ftox.2026.1812922

Figure Lengend Snippet: Physicochemical characterization of PS-C-NPLs. Panels (A) correspond to PS-C-NPLs of 30 nm, panels (B) to PS-C-NPLs of 50 nm, and panels (C) to PS-C-NPLs of 100 nm. Panels (A.1–C.1) show TEM images of fluorescently labeled NPLs, with panels (A.2–C.2) displaying the corresponding size distribution histograms. Panels (B.3,C.3) present TEM images of non-labeled NPLs, with panels (B.4,C.4) showing the respective histograms. Panel (D) summarizes TEM-derived Martin’s diameter (nm), hydrodynamic diameter (d.nm), and ζ-potential (mV) values obtained by TEM and DLS in Milli-Q water and EGM-2.

Article Snippet: Cells were cultured in EGM-2 medium (C-22011, Promocell) in collagen-coated flasks prepared with rat tail collagen I (Corning Inc., New York, NY, USA) at 5 μg/cm 2 .

Techniques: Labeling, Derivative Assay